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MSCV-beta-catenin-IRES-GFP
(Plasmid #14717)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14717 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6573
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-catenin
  • Alt name
    beta-catenin deltaGSK-KT3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII blunt (unknown if destroyed)
  • 3′ cloning site SalI/XhoI (destroyed during cloning)
  • 5′ sequencing primer pLXSN-5
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-beta-catenin-IRES-GFP was a gift from Tannishtha Reya (Addgene plasmid # 14717 ; http://n2t.net/addgene:14717 ; RRID:Addgene_14717)
  • For your References section:

    A role for Wnt signalling in self-renewal of haematopoietic stem cells. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL. Nature. 2003 May 22. 423(6938):409-14. 10.1038/nature01593 PubMed 12717450