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MSCV-IRES-GFP
(Plasmid #20672)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20672 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    MSCV
  • Backbone size (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Tag / Fusion Protein
    • IRES EGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer pLXSN5; pBABE5
  • 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG)
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is essentially pMIG and we suggest ordering it instead of plasmid 9044 as it has better yield in culture and is easier to sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-IRES-GFP was a gift from Tannishtha Reya (Addgene plasmid # 20672 ; http://n2t.net/addgene:20672 ; RRID:Addgene_20672)

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