MSCV-IRES-GFP
(Plasmid #20672)

• Purpose: (Empty Backbone)
• Depositing Lab: Tannishtha Reya
• Publication: Reya 2009 (unpublished)

Backbone

• Vector backbone: MSCV
  (Search Vector Database)
• Backbone size (bp): 6500
• Vector type: Mammalian Expression, Retroviral
• Tag / Fusion Protein:
  • IRES EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN5; pBABE5
• 3′ sequencing primer: pCDH-rev (GCATTCCTTTGGCGAGAG)

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 33 References

Depositor Comments

This plasmid is essentially pMIG and we suggest ordering it instead of plasmid 9044 as it has better yield in culture and is easier to sequence.

Information for Cloning Grade DNA (Catalog # 20672-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $95 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here