MSCV-IRES-GFP
(Plasmid #20672)

• Purpose: (Empty Backbone)
• Depositing Lab: Tannishtha Reya
• Publication: Reya 2009 (unpublished)
• Sequence Information:

  • Depositor Sequences: None.
  • Addgene Sequences: Full (1) Partial (3)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	20672	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: MSCV
  (Search Vector Database)
• Backbone size (bp): 6500
• Vector type: Mammalian Expression, Retroviral
• Tag / Fusion Protein:
  • IRES EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN5; pBABE5
• 3′ sequencing primer: pCDH-rev (GCATTCCTTTGGCGAGAG)

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Articles Citing this Plasmid:
  • 20 References

Depositor Comments

This plasmid is essentially pMIG and we suggest ordering it instead of plasmid 9044 as it has better yield in culture and is easier to sequence.