Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

MSCV IRES Luciferase
(Plasmid #18760)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18760 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV IRES Luciferase
  • Backbone size (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

Depositor Comments

To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV IRES Luciferase was a gift from Scott Lowe (Addgene plasmid # 18760 ; http://n2t.net/addgene:18760 ; RRID:Addgene_18760)