-
Purpose(Empty Backbone) Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.
-
Depositing Lab
-
Publication
-
Citations
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backboneMSCV
-
Backbone manufacturerClonetech
- Backbone size (bp) 7657
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Insert Size (bp)7657
-
Tags
/ Fusion Proteins
- IRES
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5
- 3′ sequencing primer EGFP-N
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructs for Soft-agar assay
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMSCV PIG (Puro IRES GFP empty vector) was a gift from David Bartel (Addgene plasmid # 21654 ; http://n2t.net/addgene:21654 ; RRID:Addgene_21654) -
For your References section:
Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394
