|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52114||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneMSCV IRES GFP
Modifications to backboneexpanded MCS and switched FP
Vector typeMammalian Expression, Retroviral
/ Fusion Protein
- IRES-mCherry (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN_5_primer (Common Sequencing Primers)
The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-IRES-mCherry FP was a gift from Dario Vignali (Addgene plasmid # 52114 ; http://n2t.net/addgene:52114 ; RRID:Addgene_52114)