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pMSCV-IRES-YFP II (pMIY II)
(Plasmid #52108)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52108 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV IRES GFP
  • Modifications to backbone
    expanded MCS and switched FP
  • Vector type
    Mammalian Expression, Retroviral
  • Tag / Fusion Protein
    • IRES-EYFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-YFP II (pMIY II) was a gift from Dario Vignali (Addgene plasmid # 52108 ; http://n2t.net/addgene:52108 ; RRID:Addgene_52108)
  • For your References section:

    Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263