PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14715||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameLEF-1/TCF-responsive promoter
Alt namebeta catenin reporter
Insert Size (bp)100
Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
/ Fusion Protein
- d2 eGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
d2-eGFP gene (destabilized, half-life of 2 h; Clontech) was cloned downstream of a LEF-1/TCF-responsive promoter, containing three LEF-1/TCF binding motifs and a TATA box. This cassette was then cloned into a self-inactivating lentiviral vector plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lentiviral-TOP-dGFP-reporter was a gift from Tannishtha Reya (Addgene plasmid # 14715 ; http://n2t.net/addgene:14715 ; RRID:Addgene_14715)
For your References section:A role for Wnt signalling in self-renewal of haematopoietic stem cells. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL. Nature. 2003 May 22. 423(6938):409-14. 10.1038/nature01593 PubMed 12717450