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M38 TOP-dGFP
(Plasmid #17114)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17114 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tcf/Lef sites
  • Alt name
    beta catenin reporter
  • Insert Size (bp)
    1000
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Description: This is a destabilized GFP under the control of a beta-catenin responsive promoter.

A 358-bp promoter/enhancer region of TOPFLASH, containing 4 consensus Lef binding sites and a minimal promoter, was amplified by PCR. The 94-bp minimal promoter is derived from the mouse cFos gene (Accession No. AF332140), starting 38 bp 5.

The full sequence provided by the depositing lab was created by piecing together various sequences, and is not exact. It should be used as a guide only.

TCF/LEF optimal sequence - cctttgatcttacc

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M38 TOP-dGFP was a gift from Randall Moon (Addgene plasmid # 17114 ; http://n2t.net/addgene:17114 ; RRID:Addgene_17114)