|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17114||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Growth in Bacteria
Gene/Insert nameTcf/Lef sites
Alt namebeta catenin reporter
Insert Size (bp)1000
Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe promoter was from Hans Clevers' construct, TOPFLASH.
Terms and Licenses
Articles Citing this Plasmid
Description: This is a destabilized GFP under the control of a beta-catenin responsive promoter.
A 358-bp promoter/enhancer region of TOPFLASH, containing 4 consensus Lef binding sites and a minimal promoter, was amplified by PCR. The 94-bp minimal promoter is derived from the mouse cFos gene (Accession No. AF332140), starting 38 bp 5.
The full sequence provided by the depositing lab was created by piecing together various sequences, and is not exact. It should be used as a guide only.
TCF/LEF optimal sequence - cctttgatcttacc
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M38 TOP-dGFP was a gift from Randall Moon (Addgene plasmid # 17114 ; http://n2t.net/addgene:17114 ; RRID:Addgene_17114)