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Su-Chun Zhang Lab Plasmids


The Su-Chun Zhang Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Qian K, Huang CL, Chen H, Blackbourn LW 4th, Chen Y, Cao J, Yao L, Sauvey C, Du Z, Zhang SC Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653.
Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse Model of Parkinson's Disease. Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52341hAAVS1 TALEN LeftAAVS1 TALEN Left (Synthetic)Mammalian ExpressionA simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. TALEN vector for genomic targeting JDS78 Add to Cart
52342hAAVS1 TALEN RightAAVS1 TALEN Right (Synthetic)Mammalian ExpressionA simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. TALEN vector for genomic targeting JDS78 Add to Cart
52344AAVS1-CAG-hrGFPhrGFP (Synthetic)Mammalian ExpressionA simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. AAVS1 donor vector for constitutive gene expression AAVS1-CAG Add to Cart
68407PL552Mammalian Expression, Cre/LoxEngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. Donor empty vector with floxed puromycin resistance gene expression cassette PL452 Add to Cart
68460AAVS1-Neo-CAG-Flpe-ERT2mammalian-codon-optimized Flpe recombinase fused with ERT2 (Synthetic)Mammalian Expression, Synthetic BiologyEngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene AAVS1-CAG Add to Cart
68461AAVS1-Blasticidin-CAG-Flpe-ERT2mammalian-codon-optimized Flpe recombinase fused with ERT2 (Synthetic)Mammalian Expression, Synthetic BiologyEngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene AAVS1-CAG Add to Cart
68463Cas9 sgRNA vectorMammalian Expression, CRISPREngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. Cas9 sgRNA vector for cloning gRNA cloning vector (Addgene plasmid #41824) Add to Cart
68465PAX6 sgRNA2sgRNA targeting PAX6 (Homo sapiens)Mammalian Expression, CRISPREngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. targeting PAX6 gene Cas9 sgRNA vevctor Add to Cart
68466PAX6 sgRNA7sgRNA targeting PAX6 (Homo sapiens)Mammalian Expression, CRISPREngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. targeting PAX6 gene Cas9 sgRNA vevctor Add to Cart
68467PAX6 CKO 2/7 Donor plasmidFRT flanked exon4 of PAX6 gene (Homo sapiens)Mammalian Expression, Cre/LoxEngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. donor plasmid for targeting PAX6 locus PL552 Add to Cart
68468Cas9-2A-CreCas9 and Cre recombinase (Homo sapiens)Mammalian Expression, CRISPREngineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. co-expressing Cas9 and Cre recombinase Cas9-2A-GFP Add to Cart
80945AAVS1-Pur-CAG-EGFPEGFP (Other)Mammalian ExpressionChemical Control of Grafted Human PSC-Derived Neurons in a Mouse Model of Parkinson's Disease. Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 10.1016/j.stem.2016.03.014. Epub 2016 Apr 28. human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter AAVS1 Add to Cart
80946AAVS1-Pur-CAG-mCherrymCherry (Other)Mammalian ExpressionChemical Control of Grafted Human PSC-Derived Neurons in a Mouse Model of Parkinson's Disease. Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 10.1016/j.stem.2016.03.014. Epub 2016 Apr 28. human AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoter AAVS1 Add to Cart
80947AAVS1-Pur-CAG-hM4Di-mCherryhM4Di-mCherry (Homo sapiens)Mammalian ExpressionChemical Control of Grafted Human PSC-Derived Neurons in a Mouse Model of Parkinson's Disease. Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 10.1016/j.stem.2016.03.014. Epub 2016 Apr 28. human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter AAVS1 Add to Cart
80948AAVS1-Pur-CAG-hM3Dq-mCherryhM3Dq-mCherry (Homo sapiens)Mammalian ExpressionChemical Control of Grafted Human PSC-Derived Neurons in a Mouse Model of Parkinson's Disease. Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 10.1016/j.stem.2016.03.014. Epub 2016 Apr 28. human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter AAVS1 Add to Cart