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DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.
Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP
Nucleic Acids Res. 2013 Dec 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
51545p2attNG-H11-longp2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.
51546p2attNG-H11-shortp2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).
51547p2attPCDICE donor plasmid without neural transcription factors.
51551p2attPC-FO DICE donor plasmid with the FOXA2 and OTX2 transcription factors.
51552pCMV-BxExpresses Bxb1 integrase in mammalian cells.
51553pCS-kIMammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired.
51554MR015-H11-L2-TALENExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.
51555MR015-H11-R2-TALENExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).

Antibodies from Article