|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51545||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression ; Human Targeting
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Growth instructionsThis plasmid may be difficult to grow and a strain such as MDS42 ΔrecA (Scarab Genomics C-0185) might increase yields.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2attNG-H11-long was a gift from Michele Calos (Addgene plasmid # 51545 ; http://n2t.net/addgene:51545 ; RRID:Addgene_51545)
For your References section:DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP. Nucleic Acids Res. 2013 Dec 4. 10.1093/nar/gkt1290 PubMed 24304893