FUW
(Plasmid #14882)

• Depositing Lab: David Baltimore
• Publication: Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Backbone

• Vector backbone: HR'CS-G
• Backbone manufacturer: I. Verma, Salk
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: flap-Ub promoter-WRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid pFUW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap
sequence PCR-amplified
from the HIV NLA4.3 genome, the human polyubiquitin
promoter-C (gift of L. Thiel, Amgen), and the WRE (woodchuck
hepatitis virus posttranscriptional
regulatory element) (gift of D.
Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging
vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.

Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI HpaI XhoI AscI EcoRI ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI. Unique sites for subcloning in the MCS are: BamHI, HpaI, AscI, and EcoRI.