|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11950||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3932
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)826
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The YFP-CL1 reporter was generated by inserting an oligodimer encoding the 16 amino acid CL1 degradation signal (ACKNWFSSLSHFVIHL) in enhanced GFP-C1 (BD Biosciences Clontech) using the EcoRI and BamH1 restriction sites, then the enhanced GFP open-reading frame was replaced with YFP using the Nhe1 and SspB1 restriction sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YFP-CL1 was a gift from Nico Dantuma (Addgene plasmid # 11950 ; http://n2t.net/addgene:11950 ; RRID:Addgene_11950)
For your References section:Endoplasmic reticulum stress compromises the ubiquitin-proteasome system. Menendez-Benito V, Verhoef LG, Masucci MG, Dantuma NP. Hum Mol Genet. 2005 Oct 1. 14(19):2787-99. 10.1093/hmg/ddi312 PubMed 16103128