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CD3 delta YFP
(Plasmid #11951)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11951 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3961
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD3delta-YFP
  • Alt name
    CD3 delta YFP
  • Insert Size (bp)
    1265

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

CD3 delta was PCR amplified from a plasmid, introducing flanking BglII and EcoRI sites, and cloned in Ub-R-YFP digested with BamHI and EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD3 delta YFP was a gift from Nico Dantuma (Addgene plasmid # 11951 ; http://n2t.net/addgene:11951 ; RRID:Addgene_11951)
  • For your References section:

    Endoplasmic reticulum stress compromises the ubiquitin-proteasome system. Menendez-Benito V, Verhoef LG, Masucci MG, Dantuma NP. Hum Mol Genet. 2005 Oct 1. 14(19):2787-99. 10.1093/hmg/ddi312 PubMed 16103128