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pAAV-CAG-DIO-EYFP

Plasmid

#104052

Purpose

An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.V1

Insert

EYFP

Use

AAV

Promoter

CAG

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-ATLASsnFlp (AAV5)

Viral Prep

#232352-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-DIO-ATLASsnFlp (#232352). In addition to the viral particles, you will also receive purified pAAV-DIO-ATLASsnFlp plasmid DNA. Syn-driven, ATLASsnFlp expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Promoter

Syn

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only
pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-SB

Plasmid

#228869

Purpose

PE7 connected to MLH1-SB via 2A sequence

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB

Use

CRISPR

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW

Plasmid

#14883

Purpose

3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pInt_attP1_sacB_kanR

Plasmid

#205301

Purpose

Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_N_Strep_BIRC6

Plasmid

#197972

Purpose

Mammalian cell expression of wt Strep-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

Strep

Expression

Mammalian

Mutation

L1332V

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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wt dynamin 2 pEGFP

Plasmid

#34686

Depositor

Sandra Schmid

Insert

dynamin 2 (Dnm2 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D406G, V664A, H692R and Q758R

Promoter

CMV

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-mCherry

Plasmid

#65279

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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p2attNG-H11-short

Plasmid

#51546

Purpose

p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

GFP4m

Use

Human targeting

Expression

Mammalian

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOT (p130)

Plasmid

#172997

Purpose

constitutive expression of V5-tagged AMOT (p130)

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOT (p130) (AMOT Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE-eGFP-NDP52

Plasmid

#175749

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2

Depositor

Wade Harper

Article

Heo et al Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10

Insert

NDP52/CALCOCO2 (CALCOCO2 Human)

Use

Lentiviral

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pICH-chl-roGFP2-synORP1

Plasmid

#191701

Purpose

Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

Plasmid

#104495

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, and AAV1

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pIRESneo-PA-GFP-alpha Tubulin

Plasmid

#12297

Depositor

Patricia Wadsworth

Article

Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

Insert

alpha Tubulin (TUBA1B Human)

Tags

PA-GFP

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Sox11

Plasmid

#120387

Purpose

over expression of sox11 using infection

Depositor

Igor Ulitsky

Article

Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

Insert

sox11 (Sox11 Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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ER-mScarletI

Plasmid

#137805

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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