165,839 results

1. pgRNA-humanized

   Plasmid

   #44248

   Purpose

   Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+HyPer7-NES

   Plasmid

   #136467

   Purpose

   Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Nuclear export signal from the HIV Rev protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-Tol1.zf1

   Plasmid

   #61388

   Purpose

   mRNA synthesis of tol1 transposase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   tol1.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV1)

   Viral Prep

   #100845-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Tags

   None

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

   Plasmid

   #210857

   Purpose

   Lentiviral expression vector encoding FIP200 mutant-mCherry

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   V44D/V314D/L324D/R543D/R548D

   Promoter

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-PEmax for IVT

   Plasmid

   #178113

   Purpose

   Template for in vitro transcription of PEmax

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Detailed in manuscript

   Promoter

   T7 (inactivated)

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-ZAP70

   Plasmid

   #23887

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ZAP70 (ZAP70 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-FIP200 (1-640)-msfGFP

    Plasmid

    #203546

    Purpose

    FIP200 NTD-GFP-10His mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    msfGFP-10His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TLCV2-APOBEC1-YTHmut

    Plasmid

    #178950

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTHmut-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLO-Blue

    Plasmid

    #220782

    Purpose

    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    blue chromoprotein (aeBlue)
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid

    #108542

    Purpose

    Lentivirus vector expressing Luciferase with blasticidin resistance

    Depositor

    Javier Alcudia

    Inserts

    Luciferase
    Neomycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1apha and PGK

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV RasV12 Neo (w108-1)

    Plasmid

    #22259

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available Since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcw107

    Plasmid

    #62511

    Purpose

    Gateway Expression Vector

    Depositor

    John Doench, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRE4-Azi

    Plasmid

    #105829

    Purpose

    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

    Depositor

    Irene Coin

    Article

    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

    Inserts

    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (Y37L, D182S, F183M, L186A, D265R)TyrRS

    Promoter

    PGK and U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZT-C13-R1

    Plasmid

    #62197

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Right CLYBL TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-mito-mTagBFP2

    Plasmid

    #216130

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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