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Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
57818pL-CRISPR.EFS.GFPLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven
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57819pL-CRISPR.EFS.tRFPLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven
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57821pLKO5d.EFS.SpCas9.P2A.BSDLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven
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57822pLKO5.sgRNA.EFS.GFPLentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven
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57823pLKO5.sgRNA.EFS.tRFP3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven
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57824pLKO5.sgRNA.EFS.tRFP657Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven
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57825pLKO5.sgRNA.EFS.PACLentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven
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57826pL-CRISPR.SFFV.tRFPLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven
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57827pL-CRISPR.SFFV.GFPLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven
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57828pL-CRISPR.EFS.PACLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven
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57829pL-CRISPR.SFFV.PACLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven
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58329pLKO5d.EFS.SpCas9.P2A.PACLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven
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61395pL-SFFV.Reporter.RFP657.PACLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
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