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(Plasmid #57827)


Item Catalog # Description Quantity Price (USD)
Plasmid 57827 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    S. pyogenes
  • Insert Size (bp)

Gene/Insert 2

  • Gene/Insert name
    Sp sgRNA scaffold
  • Insert Size (bp)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Gene/Insert 3

  • Gene/Insert name
  • Alt name
    Spleen Focus Forming Virus promoter
  • Species
  • Insert Size (bp)

Gene/Insert 4

  • Gene/Insert name
  • Species
  • Insert Size (bp)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains the 1.9kb stuffer from the pLKO.005 vector backbone between the BsmBI sites used for sgRNA cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL-CRISPR.SFFV.GFP was a gift from Benjamin Ebert (Addgene plasmid # 57827 ; ; RRID:Addgene_57827)
  • For your References section:

    Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. 10.1038/nbt.2951 PubMed 24952903