Purpose(Empty Backbone) Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61395||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8626
Vector typeMammalian Expression, Lentiviral
- Promoter SFFV
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer GCTTCCCGAGCTCTATAAAAGAG
- 3′ sequencing primer TGACAGGTGGTGGCAATGCC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pL-SFFV.Reporter.RFP657.PAC was a gift from Benjamin Ebert (Addgene plasmid # 61395 ; http://n2t.net/addgene:61395 ; RRID:Addgene_61395)
For your References section:Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. 10.1038/nbt.2951 PubMed 24952903