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Synchronization of secretory protein traffic in populations of cells.
Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F
Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65252Str-KDEL_ManII-SBP-EGFPsynchronize trafficking of ManII from the ER (RUSH system)
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65253Str-KDEL_ManII-SBP-mCherrysynchronize trafficking of ManII from the ER (RUSH system)
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65254Str-KDEL_ManII-SBP-tagBFPsynchronize trafficking of ManII from the ER (RUSH system)
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65255Ii-Str_ManII-SBP-EGFPsynchronize trafficking of ManII from the ER (RUSH system)
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65256Ii-Str_ManII-SBP-mCherrysynchronize trafficking of ManII from the ER
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65257Ii-Str_ManII-SBP-tagBFPsynchronize trafficking of ManII from the ER (RUSH system)
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65258pCDH_Str-KDEL_ManII-SBP-EGFPsynchronize trafficking of ManII from the ER (RUSH system)
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65259pCDH_Str-KDEL_ManII-SBP-mCherrysynchronize trafficking of ManII from the ER (RUSH system)
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65260ManII-SBP-EGFP_puromycinManII reporter only (no hook) (RUSH system)
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65261ManII-SBP-mCherry_puromycinManII reporter only (no hook) (RUSH system)
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65262Str-STIM1-NN_ST-SBP-EGFPsynchronize trafficking of ST from the ER (RUSH system)
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65263Str-STIM1-NN_ST-SBP-mCherrysynchronize trafficking of ST from the ER (RUSH system)
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65264Str-KDEL_ST-SBP-EGFPsynchronize trafficking of ST from the ER (RUSH system)
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65265Str-KDEL_ST-SBP-mCherrysynchronize trafficking of ST from the ER (RUSH system)
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65266Str-KDEL_ST-SBP-tagBFPsynchronize trafficking of ST from the ER (RUSH system)
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65267pCDH_Str-KDEL_ST-SBP-EGFPsynchronize trafficking of ST from the ER (RUSH system)
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65269Ii-Str_ST-SBP-mCherrysynchronize trafficking of ST from the ER (RUSH system)
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65270ST-SBP-EGFP_puromycinST reporter only ( no hook) (RUSH system)
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65271ST-SBP-mCherry_puromycinST reporter only ( no hook) (RUSH system)
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65272Str-KDEL_flGalT-SBP-EGFPsynchronize trafficking of GalT from the ER (RUSH system)
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65273Str-KDEL_flGalT-SBP-mCherrysynchronize trafficking of GalT from the ER (RUSH system)
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65274Str-KDEL_flGalT-SBP-tagBFPsynchronize trafficking of GalT from the ER (RUSH system)
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65275flGalT-SBP-EGFP_puromycinGalT reporter only ( no hook) (RUSH system)
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65276flGalT-SBP-mCherry_puromycinGalT reporter only ( no hook) (RUSH system)
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65277Ii-Str_ssSBP-EGFPto synchronize transport of secretory soluble GFP from the ER (RUSH system)
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65278Str-KDEL_TNF-SBP-EGFPsynchronize trafficking of TNF from the ER (RUSH system)
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65279Str-KDEL_TNF-SBP-mCherrysynchronize trafficking of TNF from the ER (RUSH system)
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65280Ii-Str_TNF-SBP-EGFPsynchronize trafficking of TNF from the ER (RUSH system)
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65282pCDH_TNF-SBP-EGFPTNF reporter only (no hook) (RUSH system)
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65283pCDH_TNF-SBP-mCherryTNF reporter only (no hook) (RUSH system)
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65284TNF-SBP-EGFP_puromycinTNF reporter only (no hook) (RUSH system)
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65285TNF-SBP-mCherry_puromycinTNF reporter only (no hook) (RUSH system)
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65286Str-KDEL_SBP-EGFP-Ecadherinsynchronize trafficking of Ecadherin from the ER (RUSH system)
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65287Str-KDEL_SBP-mCherry-Ecadherinsynchronize trafficking of Ecadherin from the ER (RUSH system)
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65288Ii-Str_SBP-EGFP-Ecadherinsynchronize trafficking of Ecadherin from the ER (RUSH system)
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65289Ii-Str_SBP-mCherry-Ecadherinsynchronize trafficking of Ecadherin from the ER (RUSH system)
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65290pCDH_SBP-EGFP-EcadherinEcadherin reporter only (no hook) (RUSH system)
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65291pCDH_SBP-mCherry-EcadherinEcadherin reporter only (no hook) (RUSH system)
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65292SBP-EGFP-Ecadherin_puromycinEcadherin reporter only (no hook) (RUSH system)
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65293SBP-mCherry-Ecadherin_puromycinEcadherin reporter only (no hook) (RUSH system)
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65294Str-KDEL_SBP-EGFP-GPIsynchronize trafficking of EGFP-GPI from the ER (RUSH system)
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65295Str-KDEL_SBP-mCherry-GPIsynchronize trafficking of mCherry-GPI from the ER (RUSH system)
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65296Ii-Str_SBP-EGFP-GPIsynchronize trafficking of EGFP-GPI from the ER (RUSH system)
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65297ii-Str_SBP-mCherry-GPIsynchronize trafficking of mCherry-GPI from the ER (RUSH system)
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65298pCDH_SBP-EGFP-GPIsynchronize trafficking of EGFP-GPI from the ER (RUSH system)
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65299pCDH_SBP-mCherry-GPIsynchronize trafficking of mCherry-GPI from the ER (RUSH system)
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65300Str-Ii_VSVG-SBP-EGFPsynchronize trafficking of VSVG from the ER (RUSH system)
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65301Str-Ii_VSVG-SBP-mCherrysynchronize trafficking of VSVG from the ER (RUSH system)
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65302VSVGwt-SBP-EGFP_puromycinVSVG reporter only (no hook) (RUSH system)
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65303Str-Ii_SBP-EGFP-Golgin84synchronize trafficking of Golgin-84 from the ER (RUSH system)
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65304Str-Ii_SBP-mCherry-Golgin84synchronize trafficking of Golgin-84 from the ER (RUSH system)
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65305Str-Golgin84_VSVG-SBP-EGFPsynchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)
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65306Str-KDEL_neomycinLuminal ER hook only (RUSH system)
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65307pCDH_Str-KDEL_neomycinLuminal ER hook only (RUSH system)
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65308Ii-Str_neomycinLuminal ER hook only (RUSH system)
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65309Ii-Str_puromycinLuminal ER hook only (RUSH system)
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65310Str-STIM1-NN_puromycinLuminal ER hook only (RUSH system)
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65311Str-STIM1-NN_neomycinLuminal ER hook only (RUSH system)
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65312Str-Ii_neomycinCytoplasmic ER hook only (RUSH system)
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65313pCDH_Str-IiCytoplasmic ER hook only (RUSH system)
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Recombinant Antibodies from Article