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Ii-Str_ST-SBP-mCherry
(Plasmid #65269)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65269 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ii-Streptavidin and truncated sialyltransferase
  • Alt name
    ST6GAL1
  • Species
    H. sapiens (human)
  • Mutation
    contains only amino acids 1 to 43 of ST
  • Entrez Gene
    ST6GAL1 (a.k.a. SIAT1, ST6GalI, ST6N)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Streptavidin Binding Protein (SBP) (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

luminal tagging

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ii-Str_ST-SBP-mCherry was a gift from Franck Perez (Addgene plasmid # 65269 ; http://n2t.net/addgene:65269 ; RRID:Addgene_65269)
  • For your References section:

    Synchronization of secretory protein traffic in populations of cells. Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F. Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928. 10.1038/nmeth.1928 PubMed 22406856