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lentiSAM v2 (Puro)
(Plasmid #92062)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92062 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lentiSAM v2
  • Backbone manufacturer
    Feng Zhang lab
  • Backbone size (bp) 13713
  • Modifications to backbone
    T2A-blasticidin was removed by digestion with Bsp1407I and EcoRI. P2A-puromycin was added by PCR amplification from lentiCRISP v2 then ligation into these restriction sites.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6 (sgRNA) and EF1a (dCas9-VP64)
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiSAM v2 (Puro) was a gift from Adam Karpf (Addgene plasmid # 92062 ; http://n2t.net/addgene:92062 ; RRID:Addgene_92062)
  • For your References section:

    Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer. Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick C, Odunsi K, Drapkin R, Zou L, Karpf AR. Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070. 10.7554/eLife.55070 PubMed 33890574