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Engineered DNA ligases with improved activities in vitro.
Wilson RH, Morton SK, Deiderick H, Gerth ML, Paul HA, Gerber I, Patel A, Ellington AD, Hunicke-Smith SP, Patrick WM
Protein Eng Des Sel. 2013 Jul;26(7):471-8. doi: 10.1093/protein/gzt024. Epub 2013 Jun 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87741pCA24N-ligaseIPTG-inducible expression of T4 DNA ligase for protein purification
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87742pCA24N-p50-ligaseIPTG-inducible expression of p50-ligase fusion for protein purification
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87743pCA24N-ligase-cTFIPTG-inducible expression of ligase-cTF fusion for protein purification
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87744pCA24N-ligase-pprAIPTG-inducible expression of ligase-pprA fusion for protein purification
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87745pCA24N-sso7d-ligaseIPTG-inducible expression of sso7d-ligase fusion for protein purification
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87746pCA24N-ligase-NLS-cTFIPTG-inducible expression of ligase-NLS-cTF fusion for protein purification
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