Engineered DNA ligases with improved activities in vitro.
Wilson RH, Morton SK, Deiderick H, Gerth ML, Paul HA, Gerber I, Patel A, Ellington AD, Hunicke-Smith SP, Patrick WM
Protein Eng Des Sel. 2013 Jul;26(7):471-8. doi: 10.1093/protein/gzt024. Epub 2013 Jun 10. PubMed Article
Wilson RH, Morton SK, Deiderick H, Gerth ML, Paul HA, Gerber I, Patel A, Ellington AD, Hunicke-Smith SP, Patrick WM
Protein Eng Des Sel. 2013 Jul;26(7):471-8. doi: 10.1093/protein/gzt024. Epub 2013 Jun 10. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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87741 | pCA24N-ligase | IPTG-inducible expression of T4 DNA ligase for protein purification |
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87742 | pCA24N-p50-ligase | IPTG-inducible expression of p50-ligase fusion for protein purification |
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87743 | pCA24N-ligase-cTF | IPTG-inducible expression of ligase-cTF fusion for protein purification |
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87744 | pCA24N-ligase-pprA | IPTG-inducible expression of ligase-pprA fusion for protein purification |
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87745 | pCA24N-sso7d-ligase | IPTG-inducible expression of sso7d-ligase fusion for protein purification |
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87746 | pCA24N-ligase-NLS-cTF | IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification |
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