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Pooled extracellular receptor-ligand interaction screening using CRISPR activation.
Chong ZS, Ohnishi S, Yusa K, Wright GJ
Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
113341pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpAExpresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins
113342pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpAExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteins
113343pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpAExpresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins
113344pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpAExpresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins
113345Membrane protein gRNA libraryContains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAs

Antibodies from Article