Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Pooled extracellular receptor-ligand interaction screening using CRISPR activation.
Chong ZS, Ohnishi S, Yusa K, Wright GJ
Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113341pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpAExpresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins
Loading...
113342pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpAExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteins
Loading...
113343pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpAExpresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins
Loading...
113344pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpAExpresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins
Loading...
113345Membrane protein gRNA libraryContains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAs
Loading...