In vivo measurement of afferent activity with axon-specific calcium imaging.
Broussard GJ, Liang Y, Fridman M, Unger EK, Meng G, Xiao X, Ji N, Petreanu L, Tian L
Nat Neurosci. 2018 Sep;21(9):1272-1280. doi: 10.1038/s41593-018-0211-4. Epub 2018 Aug 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111261 | pAAV-hSynapsin1-axon-GCaMP6m | For enriched expression of GCaMP6m in axons |
| 111262 | pAAV-hSynapsin1-axon-GCaMP6s | For enriched expression of GCaMP6s in axons |
| 112000 | pAAV-hSynapsin1-axon-GCaMP6f | For enriched expression of GCaMP6f in axons |
| 112004 | pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 | For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2 |
| 112005 | pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 | For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 |
| 112006 | pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 | For co-expression of GCaMP6s with mKate2 |
| 112007 | pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 | For co-expression of GCaMP6s with mRuby3 |
| 112008 | pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3 | For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 |
| 112010 | pAAV-hSynapsin1-FLEx-axon-GCaMP6s | For cre-dependent enriched expression of GCaMP6s in axons |