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Directed evolution improves the catalytic efficiency of TEV protease.
Sanchez MI, Ting AY
Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
135452Gal:mCherry-CRY2-uTEV1Δ(220-242)Yeast expression of uTEV1Δ fused to CRY2 and mCherry
135453Gal:mCherry-uTEV3Yeast expression of uTEV3 fused to mCherry
135454MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5Bacterial expression of TEVΔ protease
135455MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5Bacterial expression of uTEV1Δ protease
135456MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5Bacterial expression of uTEV2Δ protease
135457UAS-mCherryMammalian mCherry reporter to read out FLARE and SPARK activation
135458CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5Mammalian expression of SPARK TF component
135459CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)Mammalian expression of SPARK protease component
135460CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4Mammalian expression of uFLARE TF component
135461CMV:eGFP-CaM-uTEV1Δ(220-242)Mammalian expression of uFLARE protease component
135462Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTANeuronal expression of uFLARE TF component
135463Syn:eGFP-CaM-uTEV1Δ(220-242)Neuronal expression of uFLARE protease component
135464His6-MBP-uTEV3Bacterial expression of uTEV3 (full-length) protease
135465TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYCMembrane-anchored TF used for yeast directed evolution of TEV protease

Antibodies from Article