Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Directed evolution improves the catalytic efficiency of TEV protease.
Sanchez MI, Ting AY
Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135452Gal:mCherry-CRY2-uTEV1Δ(220-242)Yeast expression of uTEV1Δ fused to CRY2 and mCherry
Loading...
135453Gal:mCherry-uTEV3Yeast expression of uTEV3 fused to mCherry
Loading...
135454MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5Bacterial expression of TEVΔ protease
Loading...
135455MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5Bacterial expression of uTEV1Δ protease
Loading...
135456MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5Bacterial expression of uTEV2Δ protease
Loading...
135457UAS-mCherryMammalian mCherry reporter to read out FLARE and SPARK activation
Loading...
135458CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5Mammalian expression of SPARK TF component
Loading...
135459CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)Mammalian expression of SPARK protease component
Loading...
135460CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4Mammalian expression of uFLARE TF component
Loading...
135461CMV:eGFP-CaM-uTEV1Δ(220-242)Mammalian expression of uFLARE protease component
Loading...
135462Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTANeuronal expression of uFLARE TF component
Loading...
135463Syn:eGFP-CaM-uTEV1Δ(220-242)Neuronal expression of uFLARE protease component
Loading...
135464His6-MBP-uTEV3Bacterial expression of uTEV3 (full-length) protease
Loading...
135465TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYCMembrane-anchored TF used for yeast directed evolution of TEV protease
Loading...