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(Plasmid #135453)


Item Catalog # Description Quantity Price (USD)
Plasmid 135453 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pRSII 413
  • Backbone manufacturer
    Steven B. Haase
  • Backbone size w/o insert (bp) 5807
  • Total vector size (bp) 7361
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Galactose media to induce expression
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    S219V mutation improves stability , I138T/S153N/T180A mutations improve catalytic activity
  • Promoter Gal
  • Tag / Fusion Protein
    • mCherry

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal:mCherry-uTEV3 was a gift from Alice Ting (Addgene plasmid # 135453 ; ; RRID:Addgene_135453)
  • For your References section:

    Directed evolution improves the catalytic efficiency of TEV protease. Sanchez MI, Ting AY. Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7. 10.1038/s41592-019-0665-7 PubMed 31819267