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(Plasmid #135455)


Item Catalog # Description Quantity Price (USD)
Plasmid 135455 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 5479
  • Total vector size (bp) 7357
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use BL21(DE3)-RIL for protein expression. Shifting the temperature from 37C to 30C during induction maximizes the yield of soluble uTEV1Δ protease. Ampicillin for MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5; Chloramphenicol for pRIL.
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    S219V mutation improves stability, S153N mutation improves catalytic activity. Truncated form of TEV protease (low affinity)
  • Promoter tac promoter
  • Tag / Fusion Protein
    • MBP, His6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5 was a gift from Alice Ting (Addgene plasmid # 135455 ; ; RRID:Addgene_135455)
  • For your References section:

    Directed evolution improves the catalytic efficiency of TEV protease. Sanchez MI, Ting AY. Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7. 10.1038/s41592-019-0665-7 PubMed 31819267