pSK+KanaRpsL
(Plasmid #20871)

• Depositing Lab: Jiyue Zhu
• Publication: Wang et al Mol Biotechnol. 2009 Jan 22. ():.

Backbone

• Vector backbone: pBluescript SK+
• Backbone size w/o insert (bp): 2935
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Any commonly used strains
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KanamycinResistance-RpsL
• Alt name: KanaRpsL
• Alt name: streptomycin
• Species: E.coli
• Insert Size (bp): 1743
• Mutation: none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry