168,037 results

1. pCC1R

   Plasmid

   #213497

   Purpose

   Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   pcaU
   nahR
   rpaR (rpaR )
   phlF
   cinR
   vanR
   aiiA
   

   Use

   Synthetic Biology

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-C

   Plasmid

   #213496

   Purpose

   Encodes Node C of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   lambda cI 5G6GP
   mCherry
   

   Use

   Synthetic Biology

   Tags

   ASV tag and LVA tag

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD100

   Plasmid

   #212924

   Purpose

   Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SUC2 (SUC2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-NRF2-E79Q-V5-miniTurbo

   Plasmid

   #207814

   Purpose

   C-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   NRF2-E79Q-V5-miniTurbo (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Mutation

   E79Q

   Promoter

   UBC

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. T1B/pUBQ10(mTurq->mScarlet)

   Plasmid

   #195946

   Purpose

   Bxb1 target with pUBQ10 switching from mTurq to mScarlet

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   tRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
   

   Expression

   Plant

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1B1

   Plasmid

   #195944

   Purpose

   Level 1 Bxb1 cassette with constitutive promoter : pPP2AA3-Bxb1-tUBQ1

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   pPP2AA3-Bxb1-tUBQ1
   

   Expression

   Plant

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-VAChT [N425/45]

   Plasmid

   #190326

   Purpose

   Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9_CL

   Plasmid

   #166245

   Purpose

   regulated dCas9 generator

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   P112-sgRNA-term-P104-RBS1-dCas9-B15
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and Ec-TTL-P104

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]

   Plasmid

   #118807

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of NTD in full-length TDP43

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4QOX

    Plasmid

    #61959

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-CDPK4
    

    Use

    Baculovirus expression

    Tags

    MHHHHHHSSGRENLYFQG

    Mutation

    See comments

    Promoter

    Polyhedrin

    Available Since

    May 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. RP185 pUAST EGFP:Or47b

    Plasmid

    #59525

    Purpose

    N-terminal fusion of GFP to Drosophila melanogaster Or47b

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or47b (Or47b Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. RP132 pUAST EGFP:Or43a

    Plasmid

    #59523

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Or43a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or43a (Or43a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pTXB1-nbMmIgG2a-Tn5

    Plasmid

    #184288

    Purpose

    Anti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    New York Genome Center, Ivan Raimondi

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbMmIgG2a-Tn5
    

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMG-RIS

    Plasmid

    #225132

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-CAG-GFP (AAV2 trial size)

    Viral Prep

    #37825-AAV2.T

    Purpose

    Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV1)

    Viral Prep

    #162377-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pICH47761

    Plasmid

    #48003

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSGDlucV3.0

    Plasmid

    #119760

    Purpose

    dual luciferase with FMDV StopGo sequences flanking MCS

    Depositor

    John Atkins

    Article

    Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40 and T7

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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