Dual-expression system for blue fluorescent protein optimization.
Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD
Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. PubMed Article
Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD
Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 179474 | pHybrid-Electra1 | Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells |
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| 179475 | pHybrid-Electra2 | Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells |
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| 179476 | pCytERM-Electra1 | ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450 |
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| 179477 | pCytERM-Electra2 | ER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450 |
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| 179478 | pH2B-Electra1 | Nuclear labeling with blue fluorescent protein Electra1 fused to H2B |
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| 179479 | pH2B-Electra2 | Nuclear labeling with blue fluorescent protein Electra2 fused to H2B |
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| 179480 | pKeratin-Electra1 | Keratin labeling with blue fluorescent protein Electra1 |
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| 179481 | pMito-Electra1 | Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A |
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| 179482 | pElectra1-LaminA | Nuclear envelope labeling with blue fluorescent protein Electra1 |
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| 179483 | pElectra2-LaminA | Nuclear envelope labeling with blue fluorescent protein Electra2 |
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| 179484 | pPaxillin-Electra1-N1 | Expresses paxillin fused to blue fluorescent protein Electra1 |
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| 184926 | pPaxillin-Electra2 | Expresses paxillin fused to blue fluorescent protein Electra2 |
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| 184927 | pMito-Electra2 | Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A |
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| 184928 | pKeratin-Electra2 | Keratin labeling with blue fluorescent protein Electra2 |
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| 184929 | pTubuiln-Electra1 | Tubulin labeling with blue fluorescent protein Electra1 |
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| 184930 | pTubulin-Electra2 | Tubulin labeling with blue fluorescent protein Electra2 |
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| 184931 | pTubulin-mTagBFP2 | Tubulin labeling with blue fluorescent protein mTagBFP2 |
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| 184932 | pSF11-wElectra1 | Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid |
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| 184933 | pSF11-wElectra2 | Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid |
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| 184934 | pSF11-wmTagBFP2 | Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid |
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| 184935 | pAAV-CAG-Electra1-P2A-EGFP | Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells |
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| 184936 | pAAV-CAG-Electra2-P2A-EGFP | Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells |
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| 184937 | pAAV-CAG-mBlueberry2-P2A-EGFP | Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells |
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| 184938 | pAAV-CAG-EBFP2-P2A-EGFP | Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells |
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| 184939 | pCytERM-EBFP2 | ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450 |
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| 184940 | pCytERM-mTagBFP2 | ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450 |
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| 184941 | pActin-Electra1 | beta-actin labelling with blue fluorescent protein Electra1 |
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| 184942 | pActin-Electra2 | beta-actin labelling with blue fluorescent protein Electra2 |
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| 184943 | pAAV-CAG-mTagBFP2-P2A-EGFP | Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells |
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| 196113 | pTol2-nbt-Electra1-P2A-mScarlet | zebrafish expression of nbt-Electra1-P2A-mScarlet |
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| 196114 | pTol2-nbt-Electra2-P2A-mScarlet | zebrafish expression of nbt-Electra2-P2A-mScarlet |
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| 196115 | pTol2-nbt-mTagBFP2-P2A-mScarlet | zebrafish expression of nbt-mTagBFP2-P2A-mScarlet |
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