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Systematic mapping of functional enhancer-promoter connections with CRISPR interference.
Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, Kane M, Cleary B, Lander ES, Engreitz JM
Science. 2016 Sep 29. pii: aag2445.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85449TRE-KRAB-dCas9-IRES-BFPLentiviral vector expressing a KRAB-dCas9 fusion protein and BFP
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85556TRE-KRAB-dCas9-IRES-GFP2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP
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85681sgOptiLentiviral vector to express sgRNAs. The cloning site is BsmBI.
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86461pGL4.23-MYCA modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.
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120341pGL4.23-MYC-e3pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.
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120342pGL4.23-MYC-e4pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.
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120343pGL4.23-MYC-e5pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.
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