pY004 (pcDNA3.1-hFnCpf1) Citations (17)
Originally described in: Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PubMed Journal
Articles Citing pY004 (pcDNA3.1-hFnCpf1)
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| A 'new lease of life': FnCpf1 possesses DNA cleavage activity for genome editing in human cells. Tu M, Lin L, Cheng Y, He X, Sun H, Xie H, Fu J, Liu C, Li J, Chen D, Xi H, Xue D, Liu Q, Zhao J, Gao C, Song Z, Qu J, Gu F. Nucleic Acids Res. 2017 Nov 2;45(19):11295-11304. doi: 10.1093/nar/gkx783. PubMed |
| Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Kleinjan DA, Wardrope C, Nga Sou S, Rosser SJ. Nat Commun. 2017 Oct 30;8(1):1191. doi: 10.1038/s41467-017-01222-y. PubMed |
| FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Swiat MA, Dashko S, den Ridder M, Wijsman M, van der Oost J, Daran JM, Daran-Lapujade P. Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007. PubMed |
| SaCas9 Requires 5'-NNGRRT-3' PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells. Xie H, Tang L, He X, Liu X, Zhou C, Liu J, Ge X, Li J, Liu C, Zhao J, Qu J, Song Z, Gu F. Biotechnol J. 2018 Apr;13(4):e1700561. doi: 10.1002/biot.201700561. Epub 2018 Jan 10. PubMed |
| A Single Multiplex crRNA Array for FnCpf1-Mediated Human Genome Editing. Sun H, Li F, Liu J, Yang F, Zeng Z, Lv X, Tu M, Liu Y, Ge X, Liu C, Zhao J, Zhang Z, Qu J, Song Z, Gu F. Mol Ther. 2018 Aug 1;26(8):2070-2076. doi: 10.1016/j.ymthe.2018.05.021. Epub 2018 Jun 15. PubMed |
Discovery of widespread Type I and Type V CRISPR-Cas inhibitors.
Marino ND, Zhang JY, Borges AL, Sousa AA, Leon LM, Rauch BJ, Walton RT, Berry JD, Joung JK, Kleinstiver BP, Bondy-Denomy J.
Science. 2018 Sep 6. pii: science.aau5174. doi: 10.1126/science.aau5174.
PubMed
Associated Plasmids |
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E, Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
PubMed
Associated Plasmids |
| Engineering the Direct Repeat Sequence of crRNA for Optimization of FnCpf1-Mediated Genome Editing in Human Cells. Lin L, He X, Zhao T, Gu L, Liu Y, Liu X, Liu H, Yang F, Tu M, Tang L, Ge X, Liu C, Zhao J, Song Z, Qu J, Gu F. Mol Ther. 2018 Nov 7;26(11):2650-2657. doi: 10.1016/j.ymthe.2018.08.021. Epub 2018 Sep 1. PubMed |
Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, Aryee MJ, Joung JK.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
PubMed
Associated Plasmids |
Enhanced Cas12a editing in mammalian cells and zebrafish.
Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA.
Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184.
PubMed
Associated Plasmids |
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis.
Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL.
Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3.
PubMed
Associated Plasmids |
| Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells. Tang L, Yang F, He X, Xie H, Liu X, Fu J, Xi H, Lu X, Liu C, Song Z, Qu J, Zhao J, Gu F. Cell Regen (Lond). 2019 Oct 28;8(2):44-50. doi: 10.1016/j.cr.2019.08.002. eCollection 2019 Dec. PubMed |
| A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing. Chen P, Zhou J, Wan Y, Liu H, Li Y, Liu Z, Wang H, Lei J, Zhao K, Zhang Y, Wang Y, Zhang X, Yin L. Genome Biol. 2020 Mar 25;21(1):78. doi: 10.1186/s13059-020-01989-2. PubMed |
| Comprehensive optimization of a reporter assay toolbox for three distinct CRISPR-Cas systems. Chen L, Gao H, Zhou B, Wang Y. FEBS Open Bio. 2021 Jul;11(7):1965-1980. doi: 10.1002/2211-5463.13198. Epub 2021 Jun 9. PubMed |
| FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella. Cheng P, Zhang Z, Yang F, Cai S, Wang L, Wang C, Wang M, Liu Y, Fei C, Zhang L, Xue F, Gu F. Front Genet. 2021 Sep 22;12:738746. doi: 10.3389/fgene.2021.738746. eCollection 2021. PubMed |
| Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Zhou J, Chen P, Wang H, Liu H, Li Y, Zhang Y, Wu Y, Paek C, Sun Z, Lei J, Yin L. Mol Ther. 2022 Jan 5;30(1):244-255. doi: 10.1016/j.ymthe.2021.10.010. Epub 2021 Oct 20. PubMed |
| The heat is on: a simple method to increase genome editing efficiency in plants. Blomme J, Develtere W, Kose A, Arraiza Ribera J, Brugmans C, Jaraba-Wallace J, Decaestecker W, Rombaut D, Baekelandt A, Daniel Fernandez Fernandez A, Van Breusegem F, Inze D, Jacobs T. BMC Plant Biol. 2022 Mar 24;22(1):142. doi: 10.1186/s12870-022-03519-7. PubMed |
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