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Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis.
Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL
Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131009pCas9GGBackbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
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131010pFnCpf1GGBackbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.
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131011pAsCpf1GGBackbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
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131012pLsCas13aGGBackbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.
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131013pCas9FnCpf1GGBackbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
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131023pcF-1Single spacer array with spacer #1 for FnCas12a.
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131024pcF-3Single spacer array with spacer #3 for FnCas12a.
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131025pcF-2Single spacer array with spacer #2 for FnCas12a.
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131026pcF-1/3/2Three-spacer array 2 for FnCas12a.
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131027pcF-1/2/3Three-spacer array 1 for FnCas12a.
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131028pcF-2/1/3Three-spacer array 3 for FnCas12a.
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131029pcF-3/1/2Three-spacer array 4 for FnCas12a.
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131030pcF-2/3/1Three-spacer array 5 for FnCas12a.
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131031pcF-3/2/1Three-spacer array 6 for FnCas12a.
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131032pFncpf1_7_spacer_arraySeven-spacer array for FnCas12a.
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