Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis.
Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL
Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3. PubMed Article
Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL
Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131009 | pCas9GG | Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette. |
| 131010 | pFnCpf1GG | Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat. |
| 131011 | pAsCpf1GG | Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat. |
| 131012 | pLsCas13aGG | Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat. |
| 131013 | pCas9FnCpf1GG | Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats. |
| 131023 | pcF-1 | Single spacer array with spacer #1 for FnCas12a. |
| 131024 | pcF-3 | Single spacer array with spacer #3 for FnCas12a. |
| 131025 | pcF-2 | Single spacer array with spacer #2 for FnCas12a. |
| 131026 | pcF-1/3/2 | Three-spacer array 2 for FnCas12a. |
| 131027 | pcF-1/2/3 | Three-spacer array 1 for FnCas12a. |
| 131028 | pcF-2/1/3 | Three-spacer array 3 for FnCas12a. |
| 131029 | pcF-3/1/2 | Three-spacer array 4 for FnCas12a. |
| 131030 | pcF-2/3/1 | Three-spacer array 5 for FnCas12a. |
| 131031 | pcF-3/2/1 | Three-spacer array 6 for FnCas12a. |
| 131032 | pFncpf1_7_spacer_array | Seven-spacer array for FnCas12a. |