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pLsCas13aGG
(Plasmid #131012)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 131012 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    direct repeat of LsCas13a
  • Insert Size (bp)
    36

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer AGCGCTCATGAGCCCGA
  • 3′ sequencing primer TGCCGCCAGGCAAATTCTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    GFP expression cassette
  • Insert Size (bp)
    1012

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer AGCGCTCATGAGCCCGA
  • 3′ sequencing primer TGCCGCCAGGCAAATTCTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLsCas13aGG was a gift from Chase Beisel (Addgene plasmid # 131012 ; http://n2t.net/addgene:131012 ; RRID:Addgene_131012)
  • For your References section:

    Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis. Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL. Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3. 10.1038/s41467-019-10747-3 PubMed 31270316