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pET-28b-RfxCas13d-His
(Plasmid #141322)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 141322 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28b-Cas9-His
  • Backbone size w/o insert (bp) 5160
  • Total vector size (bp) 8160
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rfx-Cas13d
  • Alt name
    CasRx
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    3000
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned from pT3TS-RfxCas13d
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain) and Bazzini Lab (Stowers Institute, Kansas, MO, USA)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28b-RfxCas13d-His was a gift from Ariel Bazzini & Miguel Angel Moreno-Mateos (Addgene plasmid # 141322 ; http://n2t.net/addgene:141322 ; RRID:Addgene_141322)
  • For your References section:

    CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos. Kushawah G, Hernandez-Huertas L, Abugattas-Nuñez del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin TJ, Moran AM, Sánchez Alvarado A, Málaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA. Developmental Cell. 2020. 10.1016/j.devcel.2020.07.013