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pT3TS-RfxCas13d-HA
(Plasmid #141320)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 141320 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT3TS
  • Backbone size w/o insert (bp) 3150
  • Total vector size (bp) 6115
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rfx-Cas13d
  • Alt name
    CasRx
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    2934
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #109049 used as template as a gift from Patrick Hsu (Konermann et al., 2018).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain) and Bazzini Lab (Stowers Institute, Kansas, MO, USA)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-RfxCas13d-HA was a gift from Ariel Bazzini & Miguel Angel Moreno-Mateos (Addgene plasmid # 141320 ; http://n2t.net/addgene:141320 ; RRID:Addgene_141320)
  • For your References section:

    CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos. Kushawah G, Hernandez-Huertas L, Abugattas-Nunez Del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin TJ, Moran AM, Sanchez Alvarado A, Malaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA. Dev Cell. 2020 Sep 28;54(6):805-817.e7. doi: 10.1016/j.devcel.2020.07.013. Epub 2020 Aug 7. 10.1016/j.devcel.2020.07.013 PubMed 32768421