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CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos
Kushawah G, Hernandez-Huertas L, Abugattas-Nuñez del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin TJ, Moran AM, Sánchez Alvarado A, Málaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA
Developmental Cell. 2020.
Article

Plasmids from Article

ID Plasmid Purpose  
141320pT3TS-RfxCas13d-HAPlasmid to carry out IVT of RfxCas13d (human codon-optimized)
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141321pT3TS-RfxCas13d-NLS-HAPlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)
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141322pET-28b-RfxCas13d-HisPlasmid for bacterial expression and purification of RfxCas13d protein
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