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pC013 - Twinstrep-SUMO-huLwCas13a
(Plasmid #90097)


Item Catalog # Description Quantity Price (USD)
Plasmid 90097 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.


  • Vector backbone
  • Backbone size w/o insert (bp) 6086
  • Total vector size (bp) 9542
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    Rosetta 2(DE3)pLysS Singles
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    Synthetic; Leptotrichia wadeii
  • Insert Size (bp)
  • Tag / Fusion Protein
    • 6xHis-Twin Strep-SUMO (N terminal on insert)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rosetta 2(DE3)pLysS Singles bacteria are BL21 derivative.This strain carries an additional chloramphenicol-resistant plasmid that supplies tRNAs for 7 rare codons to enhance expression of human codon optimized genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC013 - Twinstrep-SUMO-huLwCas13a was a gift from Feng Zhang (Addgene plasmid # 90097 ; ; RRID:Addgene_90097)
  • For your References section:

    Nucleic acid detection with CRISPR-Cas13a/C2c2. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. 10.1126/science.aam9321 PubMed 28408723