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pC013 - Twinstrep-SUMO-huLwCas13a
(Plasmid #90097)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90097 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 6086
  • Total vector size (bp) 9542
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Rosetta 2(DE3)pLysS Singles
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LwCas13a
  • Alt name
    LwC2c2
  • Alt name
    Cas13a
  • Alt name
    C2c2
  • Species
    Synthetic; Leptotrichia wadeii
  • Insert Size (bp)
    3280
  • Tag / Fusion Protein
    • 6x His Tag/Twin Strep/SUMO (N terminal on insert)

Resource Information

Depositor Comments

Rosetta 2(DE3)pLysS Singles bacteria are BL21 derivative.This strain carries an additional chloramphenicol-resistant plasmid that supplies tRNAs for 7 rare codons to enhance expression of human codon optimized genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC013 - Twinstrep-SUMO-huLwCas13a was a gift from Feng Zhang (Addgene plasmid # 90097 ; http://n2t.net/addgene:90097 ; RRID:Addgene_90097)
  • For your References section:

    Nucleic acid detection with CRISPR-Cas13a/C2c2. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. 10.1126/science.aam9321 PubMed 28408723