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Nucleic acid detection with CRISPR-Cas13a/C2c2.
Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F
Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
90097pC013 - Twinstrep-SUMO-huLwCas13aTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.
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91900pC009 LshCas13a locus with targeting spacerLshCas13a locus into pACYC184 with targeting spacer
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91901pC010 LshCas13a locus with nontargeting spacerLshCas13a locus into pACYC184 with nontargeting spacer
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91903pC011 LwCas13a locus with targeting spacerLwCas13a locus into pACYC184 with targeting spacer
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91904pC012 LwCas13a locus with nontargeting spacerLwCas13a locus into pACYC184 with nontargeting spacer
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