pREDCas9
(Plasmid #71541)

• Purpose: Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
• Depositing Lab: Tao Chen
• Publication: Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30.

Backbone

• Vector backbone: pSC101ts
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Cas9
• Species: S. pyogenes

Gene/Insert 2

• Gene/Insert name: lambda red genes

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: M13pUC-Rev

Gene/Insert 3

• Gene/Insert name: plasmid curing system
• Alt name: gRNA targeting the bla gene
• Promoter: pBAD

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: pBAD-F

Resource Information

• Supplemental Documents:
  • pREDCas9 genbank
• A portion of this plasmid was derived from a plasmid made by: Cas9 gene was cloned from pCas9 vector (Addgene plasmid 42876). Lambda Red genes were cloned from pTKRed (Addgene plasmid 41062)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is used for CRISPR mediated genome editing of E. coli