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Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.
Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71538pCas9curConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
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71539pGRBfor expression of gRNA in E. coli
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71541pREDCas9Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
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