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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69973pY001 (pFnCpf1_full)Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.
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69974pY003 (pFnCpf1_delta Cas)Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.
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69975pY002 (pFnCpf1_min)Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.
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69976pY004 (pcDNA3.1-hFnCpf1)Expresses humanized FnCpf1
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69977pY005 (pcDNA3.1-hLb3Cpf1)Expresses humanized Lb3Cpf1
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69978pY006 (pcDNA3.1-hBpCpf1)Expresses humanized BpCpf1
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69979pY007 (pcDNA3.1-hPeCpf1)Expresses humanized PeCpf1
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69980pY008 (pcDNA3.1-hPbCpf1)Expresses humanized LPbCpf1
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69981pY009 (pcDNA3.1-hSsCpf1)Expresses humanized SsCpf1
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69982pY010 (pcDNA3.1-hAsCpf1)Expresses humanized AsCpf1
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69983pY011 (pcDNA3.1-hLb2Cpf1)Expresses humanized Lb2Cpf1
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69984pY012 (pcDNA3.1-hCMtCpf1)Expresses humanized CMtCpf1
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69985pY013 (pcDNA3.1-hEeCpf1)Expresses humanized EeCpf1
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69986pY014 (pcDNA3.1-hMbCpf1)Expresses humanized MbCpf1
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69987pY015 (pcDNA3.1-hLiCpf1)Expresses humanized LiCpf1
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69988pY016 (pcDNA3.1-hLbCpf1)Expresses humanized LbCpf1
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69989pY017 (pcDNA3.1-hPcCpf1)Expresses humanized PcCpf1
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69990pY018 (pcDNA3.1-hPdCpf1)Expresses humanized PdCpf1
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69991pY019 (pcDNA3.1-hPmCpf1)Expresses humanized PmCpf1
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900946-His-MBP-TEV-FnCpf1Bacterial expression plasmid for protein purification
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