Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PubMed Article
Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69973 | pY001 (pFnCpf1_full) | Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array. |
| 69974 | pY003 (pFnCpf1_delta Cas) | Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed. |
| 69975 | pY002 (pFnCpf1_min) | Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed. |
| 69976 | pY004 (pcDNA3.1-hFnCpf1) | Expresses humanized FnCpf1 |
| 69977 | pY005 (pcDNA3.1-hLb3Cpf1) | Expresses humanized Lb3Cpf1 |
| 69978 | pY006 (pcDNA3.1-hBpCpf1) | Expresses humanized BpCpf1 |
| 69979 | pY007 (pcDNA3.1-hPeCpf1) | Expresses humanized PeCpf1 |
| 69980 | pY008 (pcDNA3.1-hPbCpf1) | Expresses humanized LPbCpf1 |
| 69981 | pY009 (pcDNA3.1-hSsCpf1) | Expresses humanized SsCpf1 |
| 69982 | pY010 (pcDNA3.1-hAsCpf1) | Expresses humanized AsCpf1 |
| 69983 | pY011 (pcDNA3.1-hLb2Cpf1) | Expresses humanized Lb2Cpf1 |
| 69984 | pY012 (pcDNA3.1-hCMtCpf1) | Expresses humanized CMtCpf1 |
| 69985 | pY013 (pcDNA3.1-hEeCpf1) | Expresses humanized EeCpf1 |
| 69986 | pY014 (pcDNA3.1-hMbCpf1) | Expresses humanized MbCpf1 |
| 69987 | pY015 (pcDNA3.1-hLiCpf1) | Expresses humanized LiCpf1 |
| 69988 | pY016 (pcDNA3.1-hLbCpf1) | Expresses humanized LbCpf1 |
| 69989 | pY017 (pcDNA3.1-hPcCpf1) | Expresses humanized PcCpf1 |
| 69990 | pY018 (pcDNA3.1-hPdCpf1) | Expresses humanized PdCpf1 |
| 69991 | pY019 (pcDNA3.1-hPmCpf1) | Expresses humanized PmCpf1 |
| 90094 | 6-His-MBP-TEV-FnCpf1 | Bacterial expression plasmid for protein purification |