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pY003 (pFnCpf1_delta Cas)
(Plasmid #69974)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69974 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYC184
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 4245
  • Total vector size (bp) 8759
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FnCpf1 locus without Cas genes
  • Alt name
    Cpf1
  • Species
    Francisella tularensis subsp. novicida
  • Insert Size (bp)
    4613
  • Mutation
    Cas genes are deleted from locus
  • GenBank ID
    WP_003040289.1

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pY003 (pFnCpf1_delta Cas) was a gift from Feng Zhang (Addgene plasmid # 69974)
  • For your References section:

    Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227