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Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E, Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80338pTE4495mammalian expression MbCpf1 nuclease and Mb crRNA
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80339pTE4497mammalian expression FnCpf1 nuclease and Fn crRNA
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80443WN10150Expresses dead (908A) AsCpf1
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88903pTE4565Expresses inactive/dead, humanized MbCpf1 nuclease
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88904pTE4566Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease
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88905pTE4889Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease
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107525pTE4561Expresses Lb crRNA and mCherry in mammalian cells.
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107534pTE3330Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
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107535pTE3327Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
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107536pTE3331Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
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107537pTE3334Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
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107538pTE3329Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
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107539pTE3328Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
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107540pTE3333Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
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107541pTE3336Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
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107555pTE4999Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.
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