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Enhanced Cas12a editing in mammalian cells and zebrafish.
Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA
Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120801pET-21a_2xNLS_FnCpf1_MBP_protein_expressionProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone
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124548pET-21a_FnCpf1_2xNLS_protein_expressionProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone
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126637AsCas12a-2C-NLS in pCSDestExpresses AsCas12a-2C-NLS in mammalian cells
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126638LbCas12a-2C-NLS in pCSDestExpresses LbCas12a-2C-NLS in mammalian cell
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126639FnoCas12a-2C-NLS in pCSDestExpresses FnoCas12a-2C-NLS in mammalian cells
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126640AsCas12a-DR-cr in PBCSK-Cloning vector AsCas12a DR-crRNA for expression in mammalian cells
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126641LbCas12a-DR-cr in PBCSK-Cloning vector LbCas12a DR-crRNA for expression in mammalian cells
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126642FnoCas12a-DR-cr in PBCSK-Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells
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126643LbCas12a-DR-GC-swap-cr in pBCSK+Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells
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126644FnoCas12a-DR-GC-swap-cr in pBCSK+Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells
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