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Jason Moffat Lab Plasmids


The Jason Moffat Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J Mol Cell Proteomics. 2010 Mar 19. ():.
High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
Toronto KnockOut version 3 library (TKOv3) (unpublished)
ID Kit Hidden Extra Search Info  
1000000069Toronto KnockOut Library (TKOv1) Add to Cart
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24587pLD-puro-CnVAGateway(TM) cassetteMammalian Expression, LentiviralA lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mol Cell Proteomics. 2010 Mar 19. ():. pLKO.1 Add to Cart
24588pLD-puro-CcVAGateway(TM) cassetteMammalian Expression, LentiviralA lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mol Cell Proteomics. 2010 Mar 19. ():. pLKO.1 Add to Cart
24589pLD-puro-PGKnVAGateway(TM) cassetteMammalian Expression, LentiviralA lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mol Cell Proteomics. 2010 Mar 19. ():. pLKO.1 Add to Cart
24590pLD-hygro-EnVMGateway(TM) cassetteMammalian Expression, LentiviralA lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mol Cell Proteomics. 2010 Mar 19. ():. pLKO.1 Add to Cart
24592pLD-puro-2A-rtTA-TcVAGateway(TM) cassetteMammalian Expression, LentiviralA lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mol Cell Proteomics. 2010 Mar 19. ():. pLKO.1 Add to Cart
73310Lenti‐Cas9‐2A‐BlastCas9 (Synthetic)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter lenti CRISPR pXPR_001 Add to Cart
73311pLCKOMammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection. pLKO.1 Add to Cart
74179pLCKO_LacZ_sgRNALacZ sgRNA (Other)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting LacZ pLCKO Add to Cart
74180pLCKO_PSMD1_sgRNA_1PSMD1 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting PSMD1 pLCKO Add to Cart
74181pLCKO_PSMD1_sgRNA_5PSMD1 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting PSMD1 pLCKO Add to Cart
74184pLCKO_PSMB2_sgRNA_1PSMB2 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting PSMB2 pLCKO Add to Cart
74185pLCKO_PSMB2_sgRNA_5PSMB2 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting PSMB2 pLCKO Add to Cart
74187pLCKO_EIF3D_sgRNA_1EIF3D sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting EIF3D pLCKO Add to Cart
74188pLCKO_EIF3D_sgRNA_4EIF3D sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting EIF3D pLCKO Add to Cart
74190pLCKO_Luciferase_sgRNALuciferase sgRNA (Other)Mammalian Expression, Lentiviral, CRISPRHigh-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. lentiviral vector expressing sgRNA targeting Luciferase pLCKO Add to Cart
90294Toronto KnockOut version 3 library (TKOv3)70948 gRNAs targeting 18053 protein coding genes (4 gRNAs/gene) with 142 control gRNAs targeting EGFP LacZ and luc, 71090 total Toronto KnockOut version 3 library (TKOv3) (unpublished) Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1 lentiCRISPRv2 Add to Cart