165,839 results

1. pDEST-ORF-V1

   Plasmid

   #73637

   Purpose

   Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 1 (V1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LYN11-FRB-mcherry

   Plasmid

   #38004

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   LYN (LYN Human)
   MTOR (MTOR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.pU1a-SpCas9

   Plasmid

   #121507

   Purpose

   Expresses codon-optimized SpCas9 in mammalian cells.

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCS YFP

   Plasmid

   #91759

   Purpose

   Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Venus YFP (A206K)
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag-Tet2 CD

   Plasmid

   #72219

   Purpose

   Express flag-tagged mouse Tet2 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET2 catalytic domain.

   Promoter

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

6. amilCP_Pink chromoprotein

   Plasmid

   #117851

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP_Pink
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   64A,65C, BioBrick sites removed

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

   Plasmid

   #221359

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PxylA

   Plasmid

   #55178

   Purpose

   Xylose-inducible promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   xylA promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   xylA

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. FW102 OL2-62

   Bacterial Strain

   #53735

   Purpose

   Bacterial-two hybrid reporter strain

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Bacterial Resistance

   Kanamycin and streptomycin

   Species

   Escherichia coli

   Available Since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Splicing-targeting CRISPR-Cas9 library for human lncRNAs

    Pooled Library

    #119977

    Purpose

    The CRISPR sgRNA library was designed to target the 5' or 3' splice sites of lncRNAs in genome-scale, in order to knockout the targeted lncRNAs.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pIG-602_NY-ESO-1_TCR_RFP

    Plasmid

    #207483

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO V5 HSPA8

    Plasmid

    #19514

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 8 (HSPA8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. hFerritinL

    Plasmid

    #122653

    Purpose

    Expression of hFerritinL in bacterial cells

    Depositor

    Trevor Douglas

    Insert

    light chain ferritin, human (FTL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO-GRAB-gDA3m

    Plasmid

    #208707

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-G-Flamp1

    Plasmid

    #188567

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-IRES-GFP/CREB3L1

    Plasmid

    #214767

    Purpose

    Mammalian expression of human CREB3L1

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    CREB3L1 (CREB3L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

    Plasmid

    #221360

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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