168,037 results

1. pJet1.2 sfGFP

   Plasmid

   #117123

   Purpose

   C-terminal GFP tag

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Superfolder GFP
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   sfGFP

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ABE8.20-SpRY

   Plasmid

   #226859

   Purpose

   Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8.20-SpRY
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

   Plasmid

   #86424

   Purpose

   PCR tagging module with yeast codon optimized mTurquoise2

   Depositor

   Sue Jaspersen

   Article

   Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

   Insert

   mTurquoise2
   

   Expression

   Yeast

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HsB2AR-mCherry

   Plasmid

   #137785

   Purpose

   Visualization of the Beta-2-adrenergic receptor

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Human beta2-adrenergic receptor (ADRB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2TK 14-3-3 sigma GST

   Plasmid

   #11944

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 sigma (SFN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pENTR223-BRLF1(Rta)

   Plasmid

   #195824

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BRLF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BRLF1 (BRLF1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAUX_OL

   Plasmid

   #166246

   Purpose

   use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
   

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and BBa_J23116

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOBI C-FLAG PHGDH

   Plasmid

   #212995

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2932

    Plasmid

    #144408

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFAP2A (TFAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hs.NRAS Q61R

    Plasmid

    #83179

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. ITPR1_Halo_N_allele

    Plasmid

    #178161

    Purpose

    Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (ITPR1 Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p426_Cas9_gRNA-ARS208a

    Plasmid

    #87383

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-DIO-SPOTlight-U2GCR

    Plasmid

    #231889

    Purpose

    Cre-dependent reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Insert

    DIO-SPOTlight-U2GCR
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. E. coli K-12 MG1655_rLP5(nth-ydgR)

    Bacterial Strain

    #110245

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pJK511

    Plasmid

    #155384

    Purpose

    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. RP156 pUAST EGFP:Gr21a

    Plasmid

    #59524

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Gr21 (Gr21a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLV-hSyn1-GFP

    Plasmid

    #177810

    Purpose

    Encodes a GFP driven by human synapsin I promoter

    Depositor

    Christopher Grigsby

    Article

    Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

    Insert

    CopGFP inserted behind human synapsin I promoter (SYN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Syn1

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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