163,882 results

1. pMSCV-NLS-CBRed-d2-hygro

   Plasmid

   #139196

   Purpose

   non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBRed
   

   Use

   Luciferase and Retroviral

   Tags

   d2 PEST domain and nuclear localization signal (N…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PIA586

   Plasmid

   #104399

   Purpose

   vector for expression of single E.coli RNAP subunit

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoD
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUW-TetO-Fos

   Plasmid

   #131593

   Purpose

   doxycycline-inducible expression of mouse c-Fos in mammalian cells

   Depositor

   Filipe Pereira, Kateri Moore

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   FBJ osteosarcoma oncogene (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDmDERusk

   Plasmid

   #213702

   Purpose

   Vector for red-light-induced bacterial gene expression

   Depositor

   Andreas Moeglich

   Article

   Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-hM4D(Gi)-mCherry (AAV9)

   Viral Prep

   #50475-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pDmREDusk

   Plasmid

   #213701

   Purpose

   Vector for red-light-repressed bacterial gene expression

   Depositor

   Andreas Moeglich

   Article

   Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-mCherry-Binder

   Plasmid

   #179139

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

    Plasmid

    #124603

    Purpose

    Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Has Service

    AAV9

    Insert

    Soma-localized ChrimsonR
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREDawn-StrR-MCS

    Plasmid

    #188979

    Purpose

    For red light-induced bacterial expression of gene of interest (StrR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH41421

    Plasmid

    #50339

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. C098

    Plasmid

    #174345

    Purpose

    Template for making Nluc mRNA reporter with pA139.

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    NanoLuc luciferase
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRwB

    Plasmid

    #48734

    Purpose

    dsRed plasmid

    Depositor

    John Schiller

    Article

    Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

    Insert

    dsRed-Express
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

    Plasmid

    #130990

    Purpose

    AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-CMV-TetO2_mRuby2

    Plasmid

    #113894

    Purpose

    Mammalian (inducible) expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Ruby2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1479 pcDNA3 flag HA 14-3-3 gamma

    Plasmid

    #9000

    Depositor

    William Sellers

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only