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A mitotic chromatin phase transition prevents perforation by microtubules.
Schneider MWG, Gibson BA, Otsuka S, Spicer MFD, Petrovic M, Blaukopf C, Langer CCH, Batty P, Nagaraju T, Doolittle LK, Rosen MK, Gerlich DW
Nature. 2022 Sep;609(7925):183-190. doi: 10.1038/s41586-022-05027-y. Epub 2022 Aug 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
191761EB3-mNeonGreenExpression of mNeonGreen fused to EB3
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191762p80-Katanin-mNeonGreenExpression of mNeonGreen fused to p80-Katanin
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191763EGFP-p300 (D1399Y)Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)
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191764EGFP-p300Expression of EGFP fused to core p300 histone acetyltransferase
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191765DsRed -7eExpression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e
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191766DsRed +9eExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
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Antibodies from Article