A mitotic chromatin phase transition prevents perforation by microtubules.
Schneider MWG, Gibson BA, Otsuka S, Spicer MFD, Petrovic M, Blaukopf C, Langer CCH, Batty P, Nagaraju T, Doolittle LK, Rosen MK, Gerlich DW
Nature. 2022 Sep;609(7925):183-190. doi: 10.1038/s41586-022-05027-y. Epub 2022 Aug 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191761 | EB3-mNeonGreen | Expression of mNeonGreen fused to EB3 |
| 191762 | p80-Katanin-mNeonGreen | Expression of mNeonGreen fused to p80-Katanin |
| 191763 | EGFP-p300 (D1399Y) | Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y) |
| 191764 | EGFP-p300 | Expression of EGFP fused to core p300 histone acetyltransferase |
| 191765 | DsRed -7e | Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e |
| 191766 | DsRed +9e | Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e |