BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis synthetic biology
Wicke N, Radford D, Verrone V, Wipat A, French CE
bioRxiv 185108 Article
Wicke N, Radford D, Verrone V, Wipat A, French CE
bioRxiv 185108 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112775 | pBP-P43 | Strong promoter activity during exponential and stationary phase |
| 112776 | pBP-Pveg | Strong promoter active during sporulation and vegetative growth |
| 112777 | pBP-PliaG | Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage |
| 112778 | pBP-Pspac | Promoter repressed by LacI (not natively produced in B. subtilis 168) |
| 112779 | pBP-Pars | Promoter of ars operon. Repressed by ArsR, induced by arsenate or arsenite |
| 112780 | pBP-ORF-xylE | Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0. |
| 112781 | pTU1-A-amyEU | Upstream homology arm, amyE encodes _-amylase |
| 112782 | pTU1-D-amyED | Downstream homology arm, amyE encodes _-amylase |
| 112783 | pTU1-A-recJU | Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance |
| 112784 | pTU1-D-recJD | Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance |
| 112785 | pTU1-A-rrnOU | Upstream homology arm, rrnO-23S (23S rRNA) |
| 112786 | pTU1-D-rrnOD | Downstream homology arm, rrnO-23S (23S rRNA) |
| 112787 | pTU1-A-spsCU | Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis |
| 112788 | pTU1-D-spsCD | Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis |
| 112789 | pTU1-A-yckABU | Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis |
| 112790 | pTU1-D-yckABD | Downstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis |
| 112791 | pTU1-C-cat | Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase) |
| 112792 | pTU2-AmyE-Pveg-eGFP | Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation |