165,839 results

1. pBbS8c_ddCpf1_final

   Plasmid

   #212040

   Purpose

   Bacterial expression of the CRISPRi system for genome editing in E. coli

   Depositor

   Quanjiang Ji

   Article

   Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

   Insert

   ddCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   L-arabinose-inducible promoter PBAD

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER dCas9-TET1CDMut

   Plasmid

   #101920

   Purpose

   The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD Mut
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated TET1 catalytic domain

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. S2060

   Bacterial Strain

   #105064

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

   Bacterial Resistance

   Streptomycin and tetracycline

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pmGFP-ADAR1-p150

   Plasmid

   #117927

   Purpose

   ADAR1-p150 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR1-p150 (ADAR Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-Halo

   Plasmid

   #200197

   Purpose

   Expression for SV40 NLS

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   3X SV40 NLS-Halo
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF_1alpha

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-CL7-2NLS-SpyCas9(dS)-A22p3star

   Plasmid

   #222970

   Purpose

   Bacterial expression of A22p-fused SpyCas9 construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His-CL7-2NLS-SpyCas9(dS)-A22p3
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-NPC1

   Plasmid

   #132295

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AAV.CAG.GLPLight1

   Plasmid

   #187467

   Purpose

   Expresses GLPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV rtTA3 Blast (w756-1)

   Plasmid

   #26429

   Purpose

   3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor A3 mutant
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG/ATP8B1-HA

    Plasmid

    #209209

    Purpose

    Mammalian expression of ATP8B1

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B1 (ATP8B1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Galphai2-mTurquoise2

    Plasmid

    #69621

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai2-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRS-BG42-GFP

    Plasmid

    #220202

    Purpose

    mini-Tn7 GFP expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-N-Myc_C223S USP7

    Plasmid

    #131243

    Purpose

    Mammalian expression of catalytically inactive Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 cyst…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_BiPVe4_ChR2_mCherry

    Plasmid

    #213937

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCA/GAL4(1-93).ER.VP16

    Plasmid

    #108216

    Purpose

    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

    Depositor

    Didier Picard

    Article

    Louvion et al Gene 1993 Sep 6;131(1):129-34.

    Insert

    Gal4 DBD-ERalpha HBD-VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERalpha HBD has G400V mutation

    Promoter

    ADH1

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNTI194

    Plasmid

    #84266

    Purpose

    Expresses Avi-tagged RPL1 and humanized birA

    Depositor

    Nicholas Ingolia

    Article

    Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

    Inserts

    RPL1 (RPL10A Human, (*see below))
    humanized birA
    

    Use

    Tags

    Avi tag, Flag, Gly/Ser linker, and TEV site

    Expression

    Mammalian

    Mutation

    R33A, A34E (*see below)

    Promoter

    CMV and P2A peptide

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3532

    Plasmid

    #145008

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LIN28B (LIN28B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG1K

    Plasmid

    #71741

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid

    #104909

    Purpose

    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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